Your results are only as good as the controls you use. To be confident that your results are accurate, and to give them reproducibility, you need to have controls in place. This means making regular use of positive and negative, reagent and assay, controls in all your experiments.
Find the right control for your experiment below.
An isotype control is an antibody with the same immunoglobulin isotype (e.g., IgG) as your main primary antibody but it doesn’t bind to a specific target. Isotype controls let you account for unwanted Fc receptor interactions, which can generate background signal.
For a conjugated primary, your isotype control should be conjugated to the same reporter. For example, you’d control for a primary antibody conjugated to FITC with an isotype antibody also conjugated to FITC. You can change isotype antibodies conjugated to FITC or APC.
This product specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.
This product specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Allophycocyanin (APC).
Blocking peptides are the original antigens we use for immunization during antibody generation. This makes blocking peptides good negative reagent controls to help confirm antibody specificity. Sometimes called “immunizing peptides” or “negative control antigens,” a blocking peptide competes with, or blocks, the primary antibody.
You’ll find corresponding blocking peptides for all the antibodies in our catalog. Just search for your antibody to find the matching blocking peptide.
Find the blocking peptide in the search results right below your antibody.
On the antibody product page in the green Control Product box on the right.