Your Immunodetection

The only immunodetection assay protocol book you’ll ever need.


Better results start with better protocols

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In this free digital Protocol Book, you’ll find the protocols and troubleshooting steps we’ve used and tweaked in our work with membrane proteins for over three decades. We want you to benefit from our years of trial and error to get your assay working as quickly, and as efficiently, as possible. We won’t say “first time” because we all know how fickle biology can be!

As well as the protocols themselves, you’ll find a selection of troubleshooting guides based on questions from customers or issues we’ve overcome in our labs. It contains all the assay fixes and adjustments made so that you don’t need to scratch your head about things we’ve already solved.

There’s even space at the end of each protocol for more notes and scribbles to be made.

Finally, you’ll find QR codes that link to the online protocols. Here you can see any updates or notes we make to those, so you always have access to the most up-to-date information available.

Here’s a glimpse of what you’ll find inside:


  • Immunocytochemistry for Fixed or Live Cells: Indirect and Direct Method
  • Immunocytochemistry for Fixed Cell
  • Immunocytochemistry for Live Cells
  • Troubleshooting Immunocytochemistry


  • Immunohistochemistry for Frozen Sections: Indirect Methods
  • Indirect Immunohistochemistry
  • Immunohistochemistry for Frozen Sections: Direct Methods
  • Immunohistochemistry for Frozen Sections: Multiplex Staining
  • Immunohistochemistry for Paraffin-embedded Sections
  • Troubleshooting Immunohistochemistry

Western Blot

  • Western Blot for High Molecular Weight (HMW) Proteins
  • Western Blotting for HMW proteins
  • Troubleshooting Western Blot

Flow Cytometry

  • Flow Cytometry for Live Cells: Indirect and Direct Methods
  • Indirect Flow Cytometry
  • Direct Live Cell Flow Cytometry Using FITC/PE/APC-Conjugated Primary Antibodies
  • Troubleshooting Flow Cytometry

Blocking Peptides

  • Blocking Peptides for Western Blot
  • Blocking Peptides for Immunohistochemistry and Immunocytochemistry

Sample Preparation for Tissues

  • Enriched Membrane Fraction Preparation
    Lysate Preparation
  • Sample Preparation for Brain Synaptosomes

Sample Preparation for Cell Lines

  • Lysate Preparation Using Laemmli Sample Buffer
  • Cell Line Sample Preparation Using Mild Detergents

Why Alomone Labs?

Because we’ve been doing this for decades, with a special focus on membrane proteins, so you can rely on our expertise and commitment to your science.

And because we produce and validate everything in-house. There are no third parties. Everything you get from us comes straight from our lab to yours

Thanks for downloading our Immunodetection Protocols Book.

This is a collection of protocols we’ve used and optimized over the years and it’s a pleasure to pass them on to other scientists.

We hope you do great things with them!